Determination of the concentration of the extract from sprouted grains

Analysis of the data presented in the study showed that an increase in the concentration of solutions of extracts from sprouted grain of cereals (mg / l) leads to an increase in the values of optical density (nm). For example, an increase in the concentration of a solution with wheat extract from 0.25 mg/l to 2.9 mg/l changes the optical density of the extract solution from 0 to 3.0 nm. A similar dynamics was observed in solutions of extracts from barley, triticale and rice grains.

Introduction. Determination of the concentration of the extract from sprouted grain is of relevance in the context of the development of biotechnology and the food industry. Considering the scientifically proven high nutritional and medicinal properties of sprouted grains, accurate and accurate measurement of the concentration of their extracts can contribute to the creation of new food products and pharmaceuticals. Due to environmental trends and increased interest in healthy eating, such research is important for the introduction of effective and environmentally sustainable methods of producing healthy foods. The article presents the results of experimental studies aimed at determining the concentration of the extract from the sprouted grain in an aqueous solution by the optical density of the solution. These data can be extremely useful in biochemistry and the food industry, where the exact concentration of active substances in extracts plays a key role.

Materials and methods. The optical density of the solutions was determined by photometric method, by determining the concentration of solutions on a laboratory spectrophotometer ShimadzuUV-1900i, based on a comparison of the light transmission and light absorption capacity of the studied solutions. The use of such an advanced instrument as the ShimadzuUV-1900i spectrophotometer made it possible to conduct a study with high accuracy and obtain reliable data.

Results and conclusions. As a result of processing of experimental data, it was shown that the concentration of the studied extracts is directly dependent on the values of the optical density of solutions. This confirms the hypothesis of an inverse correlation between the concentration of extracts and the light transmission capacity of solutions. With an increase in the concentration of solutions, an increase in the optical density values is observed. This corresponds to the basic principles of photometry and indicates that the extract effectively absorbs light in the studied wavelength range. The obtained dependence graphs allow us to determine the concentration of the obtained solutions exactly according to laboratory optical density data. This result makes it possible to simplify the process of measuring the concentration of extracts in solutions and make it more effective and accessible for wide use in laboratory conditions. The analysis of the presented data showed that the concentration of the studied extracts is directly dependent on the values of the optical density of the solutions. with an increase in the concentration of solutions, an increase in the values of optical density is observed. the obtained dependence graphs allow us to determine the concentration of the obtained solutions exactly according to laboratory optical density data. The polynomial equations of the fourth, fifth and sixth degree for each culture are obtained, which most accurately describe the concentration in the obtained solutions depending on the optical density, and the coefficient of determination is in the range R2 = 0.998-0.999, which means a functional relationship between the optical density and the concentration of the studied solutions.

1. Bioactive proteins and peptides: the current state and new trends in practical use in the food industry and feed production / D. V. Grishin, O. V. Podobed, Yu. A. Gladilina, M. V. Pokrovskaya, S. S. Alexandrova, V. S. Pokrovsky, N. N. Sokolov // Vopr. Nutrition. 2017. V. 86. № 3. Pр. 19-31.

2. Danilkina V. A., Ionikhina B. C., Tikhonov V. P. A product of high nutritional value based on deep processing of plant raw materials // Materials of the Sixth International Conference "Mill-2011." M.: Pishchepromizdat, 2011. 259 p.

3. Kleimenova N. L. Study of the functional composition of sunflower oil obtained by cold pressing // Polzunovsky vestnik. 2020. № 2. Pр. 23-26.

4. Kornilova A. R., Stepycheva N. V. Increasing the biological efficiency of cosmetics by blending oils. M.: Scientific review. Medical Sciences, 2020. 83p.

5. Prospects for the use of organomineral fertilizers on crops of spring rapeseed / T. V. Zubkov, O. A. Dubrovin, D. V. Vinogradov [et al.] // Bulletin of Michurin State Agrarian University. 2020. № 4. Pр. 35-40.

6. Pilipenko T. V., Astafieva V. V., Stepanova N. Yu. Study of the qualitative characteristics of vegetable oils by various methods // Izvestia of St. Petersburg State Agrarian University. 2015. № 39. S. 90-96.

7. Planovsky A. N., Ramm V. M., Kagan S. Z. Processes and apparatuses of chemical technology. M.: Publishing House Khim. lit-ry, 1982. Pр. 49-54.

8. Method for production of beverage from sprouted wheat grains and beverage produced by this method: pat. No. 2385659 Russia MPK A23L2/38/A. A. Strannik. Application: 2008141333/13, 17.10.2008. Published: 10.04.2010

9. Shepel V. S. On the formulation of vegetable oil mixtures for cosmetic compositions. M.: Scientific review. Medical Sciences, 2020. 87 Рp.

10. Berti C., Riso P., Brusamolino A., Porrin M. Effect on appetite control of minor cereal pseudocereal products // Br. J. Nutr. 2005. № 94. Рр. 850-858.

11. Cupp-Enyard C. Sigma’s non-specific protease activity assay-casein as a substrate // Journal of Visualized Experiments. 2008. V. 19 (1). Рр. 899-899.

12. Influence of parameters of subcritical water extraction over yield of target components from grape pomace / V. Sukmanov, Y. Petrova, L. Gaceu, A. Birca, V. Zavialov, C. Popovici // Proceeding of 6th BIOATLAS Conference, Journal of EcoAgriTourism. 2016. V. 12. No 2. P. 119-133.

13. Liu J. Iodine binding property of a ternary complex consisting of starch, protein and free fatty acids // Carbohydrate Polymers. 2009. V. 75. Рр. 351-355.

14. One step rapid dispersive liquid-liquid micro-extraction with in-situ derivatization for determination of aflatoxins in vegetable oils based on high performance liquid chromatography fluorescence detection / N. Wang [et al.] // Food chemistry. 2019. V. 287. Pр. 333-337.

15. Prepatation of ethyl alcohol from grape pomace extracted by subcritical water / V. Sukmanov [et al.] // Proceeding of 6th BIOATLAS Conference, Journal of EcoAgriTourism. 2016. V. 12. No 2. Pр. 138-144.