Study of factors influencing the induction of callusogenesis in Robinia pseudoacacia L. in vitro
https://doi.org/10.32786/2071-9485-2024-03-21
Abstract
Introduction. The use of selected material is an important condition for achieving high quality and ameliorative efficiency of protective forest plantations in agroforestry. The method of cultivation of isolated tissues on artificial nutrient media under in vitro conditions is widely used in biotechnology. Development and optimization of the processes of induction and obtaining morphogenic callus play an important role not only in scientific research, but also in industrial crop production.
Objects. Robinia pseudoacacia L. and two auxins 2,4-dichlorophenoxyacetic acid (2,4-D) and indole-3-acetic acid (IAA) were selected as the object of study.
Research methods. The study was carried out on the basis of FSC Agroecology RAS in the laboratory of biotechnology. Segments of leaf, seedling, hypocotyl, and root were used as explants. Cultivation was carried out for 4 weeks on Murashige and Scoog (MS) nutrient medium supplemented with auxins 2,4-D and IAA in three different concentrations: 0.1 mg/l; 0.5 mg/l; 1 mg/l. To evaluate the effect of light conditions on the induction of callusogenesis, half of the experimental samples were cultured in the dark, the other half with a photoperiod of 16 hr. At the end of the cultivation period, morphological parameters (color, structure), induction and average fresh weight of callus were evaluated.
Results and conclusins. Based on the results of this study, it was found that R. pseudoacacia has a high capacity for callusogenesis, direct and indirect morphogenesis, and callus induction has a direct correlation with auxin concentration. The results showed that light is essential for callus formation and cultivation, darkening of callus tissue occurs faster when cultured in the dark. Hypocotyl explants and auxin 2,4-D at a concentration of 0.5 mg/l are the most suitable for induction and obtaining callus tissue of loose and medium-dense structure for further multiplication.
Supporting agencies: The studies were carried out as part of the state task of the Research and Development Institute of the Federal Scientific Center of Agroecology of the Russian Academy of Sciences РАН № 122020100427-1 "Develop the scientific basis for the conservation and reproduction of valuable genotypes of trees and shrubs in in vitro culture"
About the Authors
N. G. Fomenko
Federal State Budget Scientific Institution «Federal Scientific Centre of Agroecology, Complex Melioration and Protective Afforestation of the Russian Academy of Sciences» (FSC of Agroecology RAS)
Russian Federation
Russian Federation
Fomenko Nadezhda Gennadievna, graduate student, junior researcher at the laboratory of biotechnology
400062, Volgograd, Universitetsky Ave., 97
O. O. Zholobova
Federal State Budget Scientific Institution «Federal Scientific Centre of Agroecology, Complex Melioration and Protective Afforestation of the Russian Academy of Sciences» (FSC of Agroecology RAS)
Russian Federation
Russian Federation
Zholobova Olga Olegovna, candidate of biological sciences, leading researcher, head of the biotechnology laboratory
400062, Volgograd, Universitetsky Ave., 97
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Review
For citations:
Fomenko N.G., Zholobova O.O. Study of factors influencing the induction of callusogenesis in Robinia pseudoacacia L. in vitro. Title in english. 2024;(3 (75)):185-194. (In Russ.) https://doi.org/10.32786/2071-9485-2024-03-21
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