Dynamics of diversity of the bacterial community of cows' uterus contents in bacterial infections
https://doi.org/10.32786/2071-9485-2024-02-30
Abstract
The article shows the dynamics of intrauterine microflora in cattle with varying degrees of disease severity, calving cows were grouped according to the nature of the vaginal secretion, and a comparative analysis of the microbial communities of the uterine contents during bacterial infections was carried out. The results of these studies will help open up opportunities for the development of new and improved treatment and prevention strategies.
Introduction. In the first weeks after birth, almost all calving animals test positive for uterine bacterial contamination, but not all animals develop clinical signs of postpartum disease.
The purpose of the study is to monitor the dynamics of changes in the quantitative and qualitative composition of bacterial communities in the uterine contents of cows with bacterial infections in the postpartum period.
Object. 22 cows 2-3 calving’s, Holstein-Friesian breed. 10,563 isolates were isolated from 132 studies of uterine contents in cows with bacterial infections.
Materials and methods. Samples of the uterine contents were taken 12 hours after calving (day 1), as well as on days 3, 9, 15, 21 and 28 after birth. Intrauterine samples were collected using cytobrushes. Identification of bacterial isolates was carried out according to methods accepted in laboratory practice.
Results and conclusions. According to the results of 22 cows during six days of sampling of cow uterine contents, 132 samples were obtained, and the total number of isolates grown in aerobic culture conditions amounted to 7440, they are represented by 202 species and 76 genera of bacteria. Four weeks after calving, the number of non-pathogenic (opportunistic) isolates decreased 1.49 times from 196 (68.06%) to 132 (45.83%). At the same time, the number of pathogenic isolates isolated decreased significantly, from 82 (28.47%) to 18 (6.25%) by 4.56 times. Data from analysis of variance: P-value 0.33, F critical 5.19, Omega square 0.16, homogeneity of variance criteria: Hartley Fmax 4.00, P-value 0.98, Cochran C 0.50, P-value 0.58.
About the Authors
G. M. Firsov
Federal State Budgetary Educational Institution of Higher Professional Education "Volgograd State Agrarian University"
Russian Federation
Russian Federation
Firsov Grigory Mikhailovich, Candidate of Veterinary Sciences, Associate Professor of the Department of Veterinary and Sanitary Expertise, Contagious Diseases and Morphology,
400002, Volgograd, Universitetsky Ave., 26
A. A. Ryadnov
Federal State Budgetary Educational Institution of Higher Professional Education "Volgograd State Agrarian University"
Russian Federation
Russian Federation
Ryadnov Aleksey Anatolyevich, Doctor of Biological Sciences, Professor, Head of the Department of Veterinary and Sanitary Expertise, Contagious Diseases and Morphology,
400002, Volgograd, Universitetsky Ave., 26
T. A. Ryadnova
Federal State Budgetary Educational Institution of Higher Professional Education "Volgograd State Agrarian University"
Russian Federation
Russian Federation
Ryadnova Tamara Aleksandrovna, Candidate of Biological Sciences, Associate Professor of the Department of Veterinary and Sanitary Expertise, Contagious Diseases and Morphology,
400002, Volgograd, Universitetsky Ave., 26
Z. Ch. Morozova
Federal State Budgetary Educational Institution of Higher Professional Education "Volgograd State Agrarian University"
Russian Federation
Russian Federation
Morozova Zoya Chermenovna, Candidate of Biological Sciences, Associate Professor of the Department of Veterinary and Sanitary Expertise, Contagious Diseases and Morphology,
400002, Volgograd, Universitetsky Ave., 26
O. V. Budtuev
Federal State Budgetary Educational Institution of Higher Professional Education "Volgograd State Agrarian University"
Russian Federation
Russian Federation
Budtuev Oleg Valerievich, Candidate of Agricultural Sciences, Associate Professor of the Department of Veterinary and Sanitary Expertise, Contagious Diseases and Morphology,
400002, Volgograd, Universitetsky Ave., 26
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Review
For citations:
Firsov G.M., Ryadnov A.A., Ryadnova T.A., Morozova Z.Ch., Budtuev O.V. Dynamics of diversity of the bacterial community of cows' uterus contents in bacterial infections. Title in english. 2024;(2 (74)):248-257. (In Russ.) https://doi.org/10.32786/2071-9485-2024-02-30
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